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SacB. The DNA deletions were verified by PCR while loss of gene expression in the adeFGH, adeIJK and adeFGHadeIJK deletion mutants was confirmed using qRT-PCR. The contribution of AdeFGH and AdeIJK pumps to MDR was defined by comparing antimicrobial susceptibilities of the isogenic mutants and the parental strains. The deletion of adeIJK produced no more than eight-fold increase in susceptibility
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